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flag tagged usp14 plasmids  (Addgene inc)


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    Addgene inc flag tagged usp14 plasmids
    Figure 3. Curcumin directly binds to <t>USP14</t> in proteasome.
    Flag Tagged Usp14 Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flag-ha-usp14/Flag-HA-USP14+(Plasmid+%2322569)/pm40294856-50-0-13
    Average 91 stars, based on 10 article reviews
    flag tagged usp14 plasmids - by Bioz Stars, 2026-09
    91/100 stars

    Images

    1) Product Images from "Curcumin modulates β-catenin stabilization via targeting proteasomal deubiquitinating enzyme USP14."

    Article Title: Curcumin modulates β-catenin stabilization via targeting proteasomal deubiquitinating enzyme USP14.

    Journal: Pharmacological research

    doi: 10.1016/j.phrs.2025.107745

    Figure 3. Curcumin directly binds to USP14 in proteasome.
    Figure Legend Snippet: Figure 3. Curcumin directly binds to USP14 in proteasome.

    Techniques Used:

    Figure 4. USP14 is required for curcumin stabilization phospho-β-catenin.
    Figure Legend Snippet: Figure 4. USP14 is required for curcumin stabilization phospho-β-catenin.

    Techniques Used:

    Figure 5. Curcumin enhances USP14-mediated PBC trapping and modulates proteasome associations.
    Figure Legend Snippet: Figure 5. Curcumin enhances USP14-mediated PBC trapping and modulates proteasome associations.

    Techniques Used:

    Figure 6. Modulation of USP14 and PBC by curcumin leads to mitotic abnormalities and cancer inhibition.
    Figure Legend Snippet: Figure 6. Modulation of USP14 and PBC by curcumin leads to mitotic abnormalities and cancer inhibition.

    Techniques Used: Inhibition

    Related Articles

    other:

    Article Title: K48/K63-linked polyubiquitination of ATG9A by TRAF6 E3 ligase regulates oxidative stress-induced autophagy.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pCDNA-Atg9 K581R-V5/His This study N/A pCDNA-Atg9 K838R-V5/His This study N/A pCDNA-Atg9 K225/581R-V5/His This study N/A pCDNA-Atg9 K581/838R-V5/His This study N/A pCDNA-HA-Beclin 1 This study N/A pRK5-HA-Ubiquitin-WT Addgene RRID:Addgene_17608 pRK5-HA-Ubiquitin-K29 Addgene RRID:Addgene_22903 pRK5-HA-Ubiquitin-K48 Addgene RRID:Addgene_17605 pRK5-HA-Ubiquitin-K63 Addgene RRID:Addgene_17606 pRK5-HA-Ubiquitin-K48R Addgene RRID:Addgene_17604 pRK5-HA-Ubiquitin-K63R This study N/A pRK5-HA-Ubiquitin-K48/63R This study N/A Flag-TRAF6 Previous study (Tang et al., 2013) N/A mRFP-EGFP-LC3 Addgene RRID:Addgene_21074 pEGFP-C1-A20 Addgene RRID:Addgene_22141 Flag-TRAF6 WT Gift from Hui-Kuan Lin (Wake Forest School of Medicine, USA) N/A Flag-TRAF6 C70A Gift from Hui-Kuan Lin (Wake Forest School of Medicine, USA) N/A Flag-VPS34 Gift from Ruey-Hwa Chen (Academia Sinica, Taiwan) N/A Flag-TRABID Gift from Ruey-Hwa Chen (Academia Sinica, Taiwan) N/A GFP-UVRAG Gift from Wei Yuan Yang (Academia Sinica, Taiwan) N/A GFP-ATG14L Gift from Wei Yuan Yang (Academia Sinica, Taiwan) N/A GFP-CYLD Gift from Wei Yuan Yang (Academia Sinica, Taiwan) N/A GFP-FYVE Gift from Wei Yuan Yang (Academia Sinica, Taiwan) N/A GFP-DFCP1 Gift from Wei Yuan Yang (Academia Sinica, Taiwan) N/A Flag-HA-USP14 Addgene RRID:Addgene_22569 Software and algorithms GraphPad Prism GraphPad software http://www.graphpad.com/ ImageJ NIH software https://imagej.net/ CytExpert Software Beckman Coulter https://www.beckman.fr/flow-cytometry/ instruments/cytoflex/software

    Article Title: USP14 regulates DNA damage repair by targeting RNF168-dependent ubiquitination
    Article Snippet: EGFP-WT-SQSTM1, EGFP-1-122-SQSTM1, EGFP-123-387-SQSTM1, EGFP-387-440-SQSTM1, EGFP-D69A-SQSTM1, EGFP-I431A-SQSTM1, plasmids were a kind gift from Dr. Terje Johansen (Institute of Medical Biology, University of Tromsø, Norway), Flag-HA-USP14 was from Addgene (22569, deposited by Wade Harper).



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    Figure 3. Curcumin directly binds to USP14 in proteasome.

    Journal: Pharmacological research

    Article Title: Curcumin modulates β-catenin stabilization via targeting proteasomal deubiquitinating enzyme USP14.

    doi: 10.1016/j.phrs.2025.107745

    Figure Lengend Snippet: Figure 3. Curcumin directly binds to USP14 in proteasome.

    Article Snippet: Flag-tagged USP14 plasmids fused with mEmerald genes were generated based on mEmeraldCalreticulin-N-16 (54023, Addgene) and synthesized by GenScript Biotechnology.

    Techniques:

    Figure 4. USP14 is required for curcumin stabilization phospho-β-catenin.

    Journal: Pharmacological research

    Article Title: Curcumin modulates β-catenin stabilization via targeting proteasomal deubiquitinating enzyme USP14.

    doi: 10.1016/j.phrs.2025.107745

    Figure Lengend Snippet: Figure 4. USP14 is required for curcumin stabilization phospho-β-catenin.

    Article Snippet: Flag-tagged USP14 plasmids fused with mEmerald genes were generated based on mEmeraldCalreticulin-N-16 (54023, Addgene) and synthesized by GenScript Biotechnology.

    Techniques:

    Figure 5. Curcumin enhances USP14-mediated PBC trapping and modulates proteasome associations.

    Journal: Pharmacological research

    Article Title: Curcumin modulates β-catenin stabilization via targeting proteasomal deubiquitinating enzyme USP14.

    doi: 10.1016/j.phrs.2025.107745

    Figure Lengend Snippet: Figure 5. Curcumin enhances USP14-mediated PBC trapping and modulates proteasome associations.

    Article Snippet: Flag-tagged USP14 plasmids fused with mEmerald genes were generated based on mEmeraldCalreticulin-N-16 (54023, Addgene) and synthesized by GenScript Biotechnology.

    Techniques:

    Figure 6. Modulation of USP14 and PBC by curcumin leads to mitotic abnormalities and cancer inhibition.

    Journal: Pharmacological research

    Article Title: Curcumin modulates β-catenin stabilization via targeting proteasomal deubiquitinating enzyme USP14.

    doi: 10.1016/j.phrs.2025.107745

    Figure Lengend Snippet: Figure 6. Modulation of USP14 and PBC by curcumin leads to mitotic abnormalities and cancer inhibition.

    Article Snippet: Flag-tagged USP14 plasmids fused with mEmerald genes were generated based on mEmeraldCalreticulin-N-16 (54023, Addgene) and synthesized by GenScript Biotechnology.

    Techniques: Inhibition